Transformation of  Bacillus thuringiensis  by electroporation | Zendy

Mahillon J. | Zendy; Chungjatupornchai W. | Zendy; Decock J. | Zendy; Dierickx S. | Zendy; Michiels F. | Zendy; Peferoen M. | Zendy; Joos H. | Zendy

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Transformation of Bacillus thuringiensis by electroporation

Author(s) -

Mahillon J.,

Chungjatupornchai W.,

Decock J.,

Dierickx S.,

Michiels F.,

Peferoen M.,

Joos H.

Publication year - 1989

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1111/j.1574-6968.1989.tb03447.x

Subject(s) - bacillus thuringiensis , electroporation , replicon , transformation (genetics) , plasmid , bacillaceae , microbiology and biotechnology , bacillales , dna , strain (injury) , biology , chemistry , bacteria , genetics , gene , bacillus subtilis , anatomy

A simple and reliable method of transforming Bacillus thuringiensis is described. This protocol, based on high‐voltage electro‐transformation (electroporation) in the presence of polyethylene glycol, allows introduction of plasmid DNA in most of the Bacillus thuringiensis strains tested. Efficiencies vary between 10 2 and 10 5 transformants per μg DNA, depending on the strain or the replicon used.

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