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Using RELION software within the Scipion framework
Author(s) -
Sharov Grigory,
Morado Dustin R.,
Carroni Marta,
de la Rosa-Trevín José Miguel
Publication year - 2021
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.374
H-Index - 138
ISSN - 2059-7983
DOI - 10.1107/s2059798321001856
Subject(s) - plug in , computer science , software , modular design , pipeline (software) , flexibility (engineering) , software engineering , key (lock) , software framework , interface (matter) , software development , software construction , operating system , statistics , mathematics , bubble , maximum bubble pressure method
Scipion is a modular image‐processing framework that integrates several software packages under a unified interface while taking care of file formats and conversions. Here, new developments and capabilities of the Scipion plugin for the widely used RELION software package are presented and illustrated with an image‐processing pipeline for published data. The user interfaces of Scipion and RELION are compared and the key differences are highlighted, allowing this manuscript to be used as a guide for both new and experienced users of this software. Different on‐the‐fly image‐processing options are also discussed, demonstrating the flexibility of the Scipion framework.