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Current approaches for automated model building into cryo‐EM maps using Buccaneer with CCP‐EM
Author(s) -
Hoh Soon Wen,
Burnley Tom,
Cowtan Kevin
Publication year - 2020
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.374
H-Index - 138
ISSN - 2059-7983
DOI - 10.1107/s2059798320005513
Subject(s) - cryo electron microscopy , pipeline (software) , software , model building , computer science , data mining , interpretation (philosophy) , artificial intelligence , algorithm , chemistry , physics , programming language , biochemistry , quantum mechanics
This work focuses on the use of the existing protein‐model‐building software Buccaneer to provide structural interpretation of electron cryo‐microscopy (cryo‐EM) maps. Originally developed for application to X‐ray crystallography, the necessary steps to optimise the usage of Buccaneer with cryo‐EM maps are shown. This approach has been applied to the data sets of 208 cryo‐EM maps with resolutions of better than 4 Å. The results obtained also show an evident improvement in the sequencing step when the initial reference map and model used for crystallographic cases are replaced by a cryo‐EM reference. All other necessary changes to settings in Buccaneer are implemented in the model‐building pipeline from within the CCP‐EM interface (as of version 1.4.0).