Premium
Crystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin–antitoxin module
Author(s) -
Hadži San,
GarciaPino Abel,
Gerdes Kenn,
Lah Jurij,
Loris Remy
Publication year - 2015
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x15000746
Subject(s) - antitoxin , vibrio cholerae , duplex (building) , crystallization , dna , cholera toxin , crystallography , toxin , resolution (logic) , chemistry , biology , physics , stereochemistry , microbiology and biotechnology , bacteria , genetics , biochemistry , organic chemistry , artificial intelligence , computer science
The HigA2 antitoxin and the HigBA2 toxin–antitoxin complex from Vibrio cholerae were crystallized in complex with their operator box. Screening of 22 different DNA duplexes led to two crystal forms of HigA2 complexes and one crystal form of a HigBA2 complex. Crystals of HigA2 in complex with a 17 bp DNA duplex belong to space group P 3 2 21, with unit‐cell parameters a = b = 94.0, c = 123.7 Å, and diffract to 2.3 Å resolution. The second form corresponding to HigA2 in complex with a 19 bp duplex belong to space group P 4 3 2 1 2 and only diffract to 3.45 Å resolution. Crystals of the HigBA2 toxin–antitoxin were obtained in complex with a 31 bp duplex and belonged to space group P 4 1 2 1 2, with unit‐cell parameters a = b = 113.6, c = 121.1 Å. They diffract to 3.3 Å resolution.