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Crystallization and preliminary X‐ray analysis of the hypothetical deaminase RPB_0146 from Rhodopseudomonas palustris HaA2
Author(s) -
Zhang Guofang,
Yu Dan,
Yang Guodong,
Dong Hui,
Zhang Tongcun,
Liu Xiang
Publication year - 2014
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x14018391
Subject(s) - rhodopseudomonas palustris , crystallization , chemistry , microbiology and biotechnology , biology , organic chemistry , genetics , bacteria
RPB_0146, a putative deaminase from Rhodopseudomonas palustris HaA2, was expressed in Escherichia coli BL21 (DE3) cells and purified using a His 6 tag by Ni 2+ ‐chelating affinity chromatography for X‐ray crystallographic analysis. Diffraction‐quality crystals were grown by the hanging‐drop vapour‐diffusion method at 289 K and diffracted to a resolution of 2.44 Å using a wavelength of 1.000 Å at the Photon Factory (KEK), Japan. The crystals belonged to the orthorhombic space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 66.26, b = 123.94, c = 155.95 Å.