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Structure of d(CCCCGGTACCGGGG) 2 at 1.65 Å resolution
Author(s) -
Purushothaman Monica,
Varghese Anna,
Mandal Pradeep Kumar,
Gautham Namasivayam
Publication year - 2014
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x1401084x
Subject(s) - resolution (logic) , geology , materials science , physics , crystallography , geometry , mathematics , computer science , chemistry , artificial intelligence
The crystal structure of the tetradecanucleotide d(CCCCGGTACCGGGG) 2 has previously been reported as an A‐type double helix at a resolution of 2.5 Å in space group P 4 1 . Here, the structure of this sequence was determined at a significantly higher resolution of 1.65 Å in space group P 4 1 2 1 2. The differences in crystal packing between the former and latter are described. The crystallographic asymmetric unit consists of one tetradecanucleotide duplex that spans more than one full turn of the A‐helix. This structure allowed the unambiguous identification of solvent interactions.