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Frq2 from Drosophila melanogaster : cloning, expression, purification, crystallization and preliminary X‐ray analysis
Author(s) -
BañosMateos Soledad,
ChavesSanjuán Antonio,
Mansilla Alicia,
Ferrús Alberto,
SánchezBarrena María José
Publication year - 2014
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x14005408
Subject(s) - drosophila melanogaster , cloning (programming) , crystallization , schneider 2 cells , biology , chemistry , genetics , gene , rna , computer science , organic chemistry , programming language , rna interference
Drosophila melanogaster contains two calcium‐binding proteins, Frq1 and Frq2, in the nervous system that control the number of synapses and the probability of release. To understand the differential function of the two proteins, whose sequence is only 5% dissimilar, the crystal structures of Frq1 and Frq2 are needed. Here, the cloning, expression, purification, crystallization and preliminary crystallographic analysis of Frq2 are presented. The full‐length protein was purified using a two‐step chromatographic procedure. Two different diffracting crystal forms were obtained using a progressive streak‐seeding method and detergents.