Open AccessRadiation damage reveals promising interaction position
Author(s) -
Koch Cornelia,
Heine Andreas,
Klebe Gerhard
Publication year - 2011
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s0909049511027920
Subject(s) - protein data bank (rcsb pdb) , bromine , chemistry , bromide , covalent bond , protein crystallization , crystallography , radiation damage , synchrotron radiation , protein data bank , protein structure , radiation , stereochemistry , physics , biochemistry , crystallization , organic chemistry , optics
High‐resolution structural data of protein inhibitor complexes are the key to rational drug design. Synchrotron radiation allows for atomic resolutions but is frequently accompanied by radiation damage to protein complexes. In this study a human aldose reductase mutant complexed with a bromine‐substituted inhibitor was determined to atomic resolution [Protein Data Bank (PDB) code 3onc ]. Though the radiation dose was moderate, a selective disruption of a bromine–inhibitor bond during the experiment was observed while the protein appears unaffected. A covalent bond to bromine is cleaved and the displaced atom is not scattered throughout the crystal but can most likely be assigned as a bromide to an additional difference electron density peak observed in the structure. The bromide relocates to an adjacent unoccupied site where promising interactions to protein residues stabilize its position. These findings were verified by a second similar structure determined with considerably higher radiation dose (PDB code 3onb ).