Open AccessHalalkalibacillus sediminis sp. nov., isolated from sediment of sea cucumber culture pond
Author(s) -
Rui-Han He,
Zhenwei Liu,
Yuqi Yan,
ZongJun Du
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003927
Subject(s) - biology , sea cucumber , sediment , microbiology and biotechnology , ecology , botany , paleontology
A Gram-stain-positive, rod-shaped (0.3–0.4×1.2–2.0 µm), strictly aerobic and beige-pigmented bacterium, designated B3227 T , was isolated from the sediment of a sea cucumber culture pond in Rongcheng, China (122.2° E 36.9° N). Its biochemical characteristics analysis revealed that the cells of this bacterium were catalase-positive and oxidase-negative. Cell growth occurred at 15–45 °C (optimum, 37 °C), pH 6.5–9.0 (pH 7.5–8.0) and in the presence of 0.0–22.0 % (w/v) NaCl (6.0–9.0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain B3227 T exhibited similarities of 95.7, 95.5, 95.5 and 95.3 % to the type strains of Filobacillus milensis , Piscibacillus salipiscarius , Halalkalibacillus halophilus and Piscibacillus halophilus , respectively, and the results of physiological analyses revealed that strain B3227 T was most similar to the genus Halalkalibacillus . The cells were endospore-forming and comprised an A1-γ- meso -diaminopimelic acid-type peptidoglycan. The respiratory quinone of strain B3227 T was MK-7, and the dominant fatty acids were anteiso-C 15 : 0 and anteiso-C 17 : 0 . The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content was 38.7 mol%. The average nucleotide identity values between strain B3227 T and H. halophilus JCM 14192 T (ANIb 69.5%, ANIm 84.2 %) and F. milensis JCM 12288 T (ANIb 70.1 %, ANIm 84.1 %) were below the cut-off level (95–96 %) for species delineation. The results of kegg analysis revealed that strain B3227 T could biosynthesize shikimate acid, a base compound for the formulation of the swine flu drug. Based on its morphological and physiological properties, as well as phylogenetic distinctiveness, strain B3227 T should be placed into the genus Halalkalibacillus as a representative of a new species, for which the name Halalkalibacillus sediminis sp. nov. is proposed. The type strain is B3227 T (=KCTC 33093 T =MCCC 1H00193 T ).