Roseomonas wenyumeiae sp. nov., isolated from faeces of Tibetan antelopes (Pantholops hodgsonii) on the Qinghai–Tibet Plateau

Two Gram-stain-negative, catalase- and oxidase-positive, non-spore-forming, aerobic, motile, flagellated, and coccus-shaped strains (Z23 T and Z24) were isolated from faeces of Tibetan antelopes ( Pantholops hodgsonii ) on the Qinghai-Tibet Plateau, PR China. Results of the morphological, biochemical, and phylogenetic studies indicated that they were similar to each other, but distinct from existing species of the genus Roseomonas . The proposed type strain, Z23 T , had 97.8, 97.1 and 96.8 % 16S rRNA similarity to Roseomonas ludipueritiae DSM 14915 T , Roseomonas aerofrigidensis JCM 31878 T and Roseomonas aerophila KACC 16529 T . Results from further phylogenetic analyses based on the 16S rRNA gene and 857 core genes indicated that the two strains were members of Roseomonas , but clearly separated from the currently recognized species. Strains Z23 T had 43.8 %, 25.0 % DNA-DNA relatedness and 91.2, 81.3 % ANI values with R. ludipueritiae DSM 14915 T and R. aerophila KACC 16529 T . The genomic DNA G+C content of strain Z23 T was 68.6 mol%. The major cellular fatty acids of strain Z23 T were C 18 : 1 ω7 c and/or C 18 : 1 ω6 c and C 19 : 0 cyclo ω 8 c . The cell-wall sugars included glucose, rhamnose and ribose. Q-10 was the sole respiratory quinone, and spermidine was the major polyamine component. Polar lipids present in strain Z23 T were phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid, phosphatidylglycerol, three aminolipids, two phospholipids and two unidentified lipids. Based on the distinct differences from other Roseomonas species judged from the genotypic and phenotypic data, a novel species represented by Z23 T and Z24, Roseomonas wenyumeiae sp. nov., is proposed. The type strain is Z23 T (=CGMCC 1.16540 T =DSM 106207 T ).