Open AccessP85gag-mos Encoded by ts110 Moloney Murine Sarcoma Virus: Rapid at the Restrictive Temperature
Author(s) -
Larry H. Stanker,
Gary E. Gallick,
Jacqueline Peltier Horn,
Ralph B. Arlinghaus
Publication year - 1983
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-64-10-2203
Subject(s) - clone (java method) , biology , virology , cell culture , transformation (genetics) , cell , sarcoma , virus , phenotype , strain (injury) , microbiology and biotechnology , cancer research , genetics , gene , medicine , pathology , anatomy
The stability of P85gag-mos, encoded by ts110 Moloney murine sarcoma virus, was studied. P85gag-mos was found to turn over more rapidly at 39 degrees C than 33 degrees C in two different cell lines. One of these is a non-producer cell clone (6m2) which appears only to express P85 at 33 degrees C. The other cell line (206-2IC) produces P85gag-mos at both 33 degrees C and 39 degrees C, although in lower amounts at the higher temperature. Both cell lines exhibit the normal morphological phenotype following a shift to the restrictive temperature. These data suggest that the presence of P85gag-mos alone is insufficient to cause transformation, and that some function(s) associated with the molecule may be heat-labile.