Detection and typing of human papillomaviruses present in fixed and stained archival cervical smears by a consensus polymerase chain reaction and direct sequence analysis allow the identification of a broad spectrum of human papillomavirus types | Zendy

H. L. Smits | Zendy; L. M. Tieben | Zendy; Steven P. TjongAHung | Zendy; Maarten F. Jebbink | Zendy; R. P. Minnaar | Zendy; C. L. Jansen | Zendy; Jan ter Schegget | Zendy

Open Access

Detection and typing of human papillomaviruses present in fixed and stained archival cervical smears by a consensus polymerase chain reaction and direct sequence analysis allow the identification of a broad spectrum of human papillomavirus types

Author(s) -

H. L. Smits,

L. M. Tieben,

Steven P. TjongAHung,

Maarten F. Jebbink,

R. P. Minnaar,

C. L. Jansen,

Jan ter Schegget

Publication year - 1992

Publication title -

journal of general virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.55

H-Index - 167

eISSN - 1465-2099

pISSN - 0022-1317

DOI - 10.1099/0022-1317-73-12-3263

Subject(s) - biology , polymerase chain reaction , virology , typing , primer (cosmetics) , papillomaviridae , papanicolaou stain , human papillomavirus , cervical cancer , genetics , medicine , gene , cancer , chemistry , organic chemistry

DNA well suited for polymerase chain reaction (PCR) amplification was purified from archival Papanicolaou smears. The detection of a wide range of human papillomavirus (HPV) types was made possible using a HPV-specific consensus primer pair, and typing was conveniently done by direct sequence analysis of the PCR product. The method could be of unique value in longitudinal and cross-sectional studies aimed at answering a number of fundamental pathological and epidemiological questions regarding HPV infection of the genital tract.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research