A Physical Map of the Pieris rapae Granulosis Virus Genome | Zendy

Kathleen G. Dwyer | Zendy; Robert R. Granados | Zendy

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A Physical Map of the Pieris rapae Granulosis Virus Genome

Author(s) -

Kathleen G. Dwyer,

Robert R. Granados

Publication year - 1987

Publication title -

journal of general virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.55

H-Index - 167

eISSN - 1465-2099

pISSN - 0022-1317

DOI - 10.1099/0022-1317-68-5-1471

Subject(s) - autographa californica , ecori , biology , pieris rapae , genome , virology , bamhi , trichoplusia , restriction enzyme , nuclear polyhedrosis virus , genetics , southern blot , virus , restriction map , gene , noctuidae , nucleic acid sequence , spodoptera , recombinant dna , lepidoptera genitalia , botany

SummaryA physical map for the genome of Pieris rapae (imported cabbageworm) granulosis virus (PrGV) was prepared using the restriction endonucleases BamHI, EcoRI, HindIII, KpnI, SacII and ScaI. The size of the genome was estimated to be 106.5 kbp. PrGV DNA sequences homologous to Trichoplusia ni GV granulin gene sequences and to some Autographa californica nuclear polyhedrosis virus EcoRI fragment sequences were located by Southern blotting. Linear organization of the two baculoviruses was compared.

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