Open AccessTHE GENERATION OF FUNCTIONALLY DIFFERENTIATED, THREE-DIMENSIONAL HEPATIC TISSUE FROM TWO-DIMENSIONAL SHEETS OF PROGENITOR SMALL HEPATOCYTES AND NONPARENCHYMAL CELLS
Author(s) -
Kohei Ogawa,
Erin R. Ochoa,
Jeffrey T. Borenstein,
Kôichi Tanaka,
Joseph P. Vacanti
Publication year - 2004
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/01.tp.0000131153.78169.24
Subject(s) - bile duct , bone canaliculus , pathology , tissue engineering , hepatocyte , progenitor cell , in vivo , biology , microbiology and biotechnology , chemistry , stem cell , medicine , in vitro , biochemistry , genetics
The authors' laboratory has investigated tissue engineering of the liver as a novel approach for treating end-stage liver disease. Fabrication of thick, viable, three-dimensional liver tissue is limited by the lack of vascularity in the tissue-engineered constructs. To overcome this limitation, the authors fabricated three-dimensional, vascularized liver tissue in vivo from two-dimensional cell sheets created from small hepatocytes (SHC) and nonparenchymal cells (NPC) implanted into rat omentum.