Open AccessT-Cell Activation and Memory Phenotypes in Cerebrospinal Fluid During HIV Infection
Author(s) -
Jutta K. Neuenburg,
Tracey A. Cho,
Annelie Nilsson,
Barry M. Bredt,
Samuel J. Hebert,
Robert M. Grant,
Richard W. Price
Publication year - 2005
Publication title -
journal of acquired immune deficiency syndromes
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.162
H-Index - 157
eISSN - 1944-7884
pISSN - 1525-4135
DOI - 10.1097/01.qai.0000155036.03004.a0
Subject(s) - cerebrospinal fluid , flow cytometry , cd38 , cd8 , immunology , t cell , cytotoxic t cell , phenotype , biology , medicine , immune system , pathology , in vitro , microbiology and biotechnology , stem cell , biochemistry , cd34 , gene
We characterized T cell phenotypes in 74 paired blood and cerebrospinal fluid (CSF) samples of HIV-infected and uninfected persons using four-color flow cytometry. CD4+ and CD8+ T cells subsets were further characterized by identifying activated/resting and memory/naive subsets in CSF and blood using the markers CD38/HLA-DR and CD45RA/CD62L, respectively. With and without HIV-infection, the proportion of CD4+ T cells and memory T cells among T cells in CSF was higher compared to blood. In HIV-infection, activated CD4+ and CD8+ T cells in CSF were more abundant than in uninfected controls. As expected, combination antiretroviral therapy (ART) reduced T cell activation in CSF and blood.