Open AccessCHANGES IN CELL SURFACE GLYCOSYLATION IN ??1,3-GALACTOSYLTRANSFERASE KNOCKOUT AND ??1,2-FUCOSYLTRANSFERASE TRANSGENIC MICE
Author(s) -
Trixie A. Shinkel,
Chao-Guang Chen,
Evelyn Salvaris,
Timothy R. Henion,
Helen Barlow,
Uri Galili,
Martin J. Pearse,
Anthony J. F. d’Apice
Publication year - 1997
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-199707270-00003
Subject(s) - epitope , genetically modified mouse , fucosyltransferase , microbiology and biotechnology , antigen , biology , galactosyltransferase , transgene , glycosylation , lectin , neuraminidase , biochemistry , enzyme , immunology , gene
Inactivation of the alpha1,3-galactosyltransferase (GalT) gene by homologous recombination (knockout [KO] mice) and competition for the enzyme's N-acetyllactosamine substrate by transgenically expressed alpha1,2-fucosyltransferase (H-transferase) are two genetic approaches to elimination of the Gal alpha1,3Gal (alphaGal) epitope, which is the major xenoantigen in pigs against which humans have preformed antibodies. Such genetic manipulations often have unpredictable results.