Open AccessINHIBITION OF RAT MIXED LYMPHOCYTE PANCREATIC ISLET CULTURES WITH ANTI-Ia IMMUNOTOXIN
Author(s) -
Shizuru Ja,
Sundaram Ramakrishnan,
Thomas A. Hunt,
Merrell Rc,
Fathman Cg
Publication year - 1986
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-198612000-00016
Subject(s) - immunotoxin , allotransplantation , islet , transplantation , cytotoxicity , mixed lymphocyte reaction , lymphocyte , in vitro , cancer research , immunology , biology , endocrinology , medicine , insulin , immune system , t cell , biochemistry
Studies reported here indicate that an anti-Ia immunotoxin can eliminate the allostimulatory subpopulation of cells present within the islets of Langerhans without damaging the hormone-secreting cells. Such studies made use of an in vitro correlate of transplantation rejection, the mixed lymphocyte islet cell (MLIC) reaction. Using the MLIC, it was demonstrated that an anti-Ia immunotoxin removed cells capable of stimulating the MLIC in a dose-dependent fashion without altering the hormone-secreting functions of the remaining cells when challenged with glucose and theophylline. These studies suggest the feasibility of using such anti-Ia immunotoxins in islet allograft transplantation models to circumvent problems inherent in complement-mediated cytotoxicity, a previously documented effective form of inducing islet allotransplantation tolerance.