Open AccessBLOCKING VERSUS CYTOTOXIC ANTIBODY IN HL-A- AND MIXED LYMPHOCYTE CULTURE-IDENTICAL AND NONIDENTICAL HUMAN RENAL TRANSPLANT RECIPIENTS
Author(s) -
Joshua Miller,
J Lifton,
Faye Rood,
Brack G. Hattler
Publication year - 1975
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-197507000-00009
Subject(s) - cytotoxic t cell , blocking antibody , blocking (statistics) , lymphocyte , antibody , immunology , cytotoxicity , transplantation , mixed lymphocyte reaction , kidney transplantation , medicine , renal transplant , kidney , immune system , biology , t cell , in vitro , biochemistry , statistics , mathematics
Blocking antibody directed against donor responding cells has been found to develop in HL-A-mixed lymphocyte culture-identical donor-recipient combinations after renal transplantation in which splenectomy was not performed. Cytotoxic antibody associated with rejection episodes and detected only by the discriminatory mixed lymphocyte culture developed in other HL-A-mixed lymphocyte culture-identical combinations as well. It disappeared as blocking antibody appeared and the post-transplant course became uneventful. In addition, both cytotoxic and blocking activity were shown to develop and coexist in a patient who received two successive renal transplants: cytotoxic against donor 1 (kidney rejected), and blocking against donor 2 (kidney accepted). These findings are taken to be strong evidence in favor of qualitatively demonstrable separate antibody molecules dealing with two separate functions, i.e., cytotoxicity versus enhancement or immunoregulation.