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The Role of CIP4 in SH‐SY5Y Cell Proliferation and Differentiation
Author(s) -
Suthivanich Phichaya,
Saengsawang Witchuda
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.877.14
Subject(s) - microbiology and biotechnology , biology , cell growth , cellular differentiation , neurogenesis , cell , biochemistry , gene
Cdc42‐interacting protein 4 (CIP4) is a protein in the F‐BAR family, which plays important roles in various cellular events including endocytosis and membrane protrusion in several cell types. CIP4 has been shown to coordinate membrane dynamics and cytoskeletal reorganization in several cell types. CIP4 is expressed in several tissues including the developing brain. However, the role of CIP4 in the brain is still unclear. Here we showed that CIP4 was highly expressed in mouse brain during an early stage of development (embryonic day 10.5), which is a period that neural progenitor cells actively proliferate and differentiate. We then determined the roles of CIP4 during cell proliferation and differentiation using neuroblastoma (SH‐SY5Y) cells as a model. We showed that CIP4 level was significantly increased in differentiated cells, which was induced by retinoic acid compared with un‐differentiated cells (proliferating cells). Overexpression of CIP4 resulted in a decrease in cell proliferation. In addition, CIP4 knockdown completely abolished the ability of retinoic acid to induce differentiation of SH‐SY5Y cells. Together these results suggest that CIP4 may play an important role during an early stage of brain development, possibly by inhibiting cell proliferation and promoting cell differentiation. Support or Funding Information This work was supported by Faculty of Science, Mahidol University and a grant from Mahidol University to WS.