Interaction between Protease Resistant Potato Protein and Intestinal Membrane

Potato has been known to offers protection against colon cancer, improves glucose tolerance and insulin sensitivity, and lowers plasma cholesterol and triglyceride concentrations. Potato proteins contain protease inhibitors, which are potentially effective appetite suppressor to increases satiety, and possibly even reduce fat storage. As our previous studies suggest that potato proteins are resistant to digestive proteases and interact with intestinal endothelial cells, we hypothesized that the protease resistant potato proteins might interact with the small intestinal mucosa layer, then, trigger certain functional signals for the satiety control and nutrient absorption. To study the function of protease resistant potato proteins on intestinal mucosa, the interacting proteins between potato and small intestinal membrane were isolated by affinity purification and identified by mass spectrometric analysis. To reduce the nonspecific binding intestinal membrane, proteins was fractionation by fractionation sucrose density gradient centrifugation. Also, cell signaling triggered by potato proteins to the intestinal cell line, Caco‐2, were accessed by phosphoproteomic analysis.