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The Effect of Doxorubicin on Mitochondrial Fusion and Fission Markers in the Kidney
Author(s) -
DirksNaylor Amie,
Kouzi Samir,
Humphrey Morgan,
Phan Diep,
Whitt Stephanie,
Gibson Jacob,
Luu Jacqueline
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.1036.3
Subject(s) - saline , doxorubicin , kidney , pharmacology , mitochondrial fission , toxicity , medicine , mitochondrion , chemistry , chemotherapy , biochemistry
Doxorubicin (DOX) is an effective chemotherapeutic agent, but is known to cause significant cardiac and hepatic toxicity. However, there has not been significant research into the effects of DOX on the kidneys. The purpose of this study was to determine if acute DOX treatment affected the content of the primary regulators of mitochondrial fusion and fission. Six‐week old F344 rats were randomly divided into two groups and injected with 20 mg/kg of DOX or saline. Once treated, the animals were fasted with no food or water until sacrifice 24 hours later. Upon sacrifice, kidneys were harvested and stored at ‐80°C for future analysis. MFN‐1, MFN‐2, Fis‐1, OPA‐1 and DRP‐1 were analyzed by Standard Western protocol. Results showed no significant difference in the content of these mitochondrial morphology regulators between the animals treated with DOX and those treated with saline. The results suggest that acute Dox treatment may not affect renal mitochondrial morphology, however further studies including microscopy techniques are required to confirm this conclusion.