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Using a yeast‐2‐hybrid system to map chemotactic protein‐protein interactions in Epulopiscium sp. type b (539.5)
Author(s) -
Conrad Arielle,
Piefer Andrew
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.539.5
Subject(s) - chemotaxis , methyltransferase , gene , yeast , fusion protein , multicellular organism , biology , plasmid , two hybrid screening , microbiology and biotechnology , genetics , chemistry , biochemistry , recombinant dna , methylation , receptor
Chemotaxis is the process by which certain single and multicellular organisms direct their movement based on chemical signals found in their environment. Epulopiscium sp type b (Epulo) is an extremely large gram positive bacterium, whose chemotactic pathway has not yet been fully explored. The purpose of this study is to expand the knowledge base of the Epulo chemotactic pathway by examining the interactions that take place between the methyltransferase, a putative methyltransferase/methylesterase fusion protein, and the methyl accepting chemotaxis proteins. PCR was used to isolate the genes that encode these proteins from purified Epulo DNA. Subsequently, these genes were subcloned into yeast expression vectors for 2‐hybrid protein interaction studies.