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Prostaglandin E2 signaling plays an important role in the regulation of the cell cycle progression in C2C12 myoblasts
Author(s) -
Mo Chenglin,
Vallejo Julian,
Isaacson Janalee,
Yang Zhipeng,
Wetmore Lori,
Allen Tara,
Igwe Orisa,
Brotto Marco
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1152.18
Subject(s) - c2c12 , myogenesis , cell cycle , myocyte , microbiology and biotechnology , cell growth , biology , cell , cellular differentiation , endocrinology , chemistry , medicine , biochemistry , gene
Skeletal muscle myogenesis is a multiple‐stage process, including quiescent satellite cells activation, myoblasts proliferation, withdrawal from the cell cycle, and myogenic differentiation to form multinucleated myotubes. We recently demonstrated that PGE 2 signaling is critical in regulation of myogenic differentiation. However, the effect of PGE 2 signaling on myoblast proliferation cycle and withdrawal from the cell cycle has not been studied. For this purpose, we employed flow cytometry to analyze the cell cycle of C2C12 myoblasts following treatment with the specific EP1 and EP4 inhibitors, SC 51322 and L161,982, respectively. C2C12 myoblasts were firstly synchronized in G0/G1 phase by serum starvation, followed by treatment with SC51322 and L161,982. Approximate 48% of myoblasts stayed in G0/G1 phase in the control group, while the percentage increased to 65% and 63% after SC51322 and L161,982 treatments for 12h. After 24h, significant inhibition on C2C12 proliferation was observed for both EP1 and EP4 inhibitors. However, the same treatment did not affect C2C12 myoblasts cell cycle withdrawal after 24h of differentiation. Our data demonstrated that PGE 2 signaling is important for cell proliferation and cell cycle progression, but not required for cell cycle withdrawal prior to early differentiation.