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ssDNA Aptamer guided “nanobomb” specific for cancer cells
Author(s) -
Lu Wentong,
LukianovaHleb Ekaterina Y,
Parekh Parag,
Lapotko Dmitri O,
Zu Youli
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1088.12
Subject(s) - aptamer , colloidal gold , cancer cell , conjugated system , nanotechnology , chemistry , nanoparticle , systematic evolution of ligands by exponential enrichment , biophysics , flow cytometry , cancer , dna , materials science , microbiology and biotechnology , biochemistry , biology , rna , polymer , genetics , organic chemistry , gene
Plasmonic nanobubbling phenomenon generated by clusters of gold nanoparticles under a short laser irradiation shows a promising therapeutic approach, as a “nanobomb”, for cancers treatemtn. In response to laser irradiatino, plasmonic nanobubbles can expand to maximal volume and rapidly break down to form an exploration within the targeted cells and kill cancer cells. To formulate a targeting gold nanoparticle and generate cancer cell‐specific “nanobom”, a ssDNA‐based aptamer with 31 nucleic acids, which is specific for the cancer cells, was developed in our lab by using a unique hybrid SELEX technique. Flow cytometry analysis showed that the developed ssDNA aptamers specifically bound to the target cancer cells at a final concentration of ≤10 nM. Subsequently, the ssDNA aptamers chemically conjugated on to the surface of the synthesized gold sphere nanoparticles, which are 60nm in diameter. Cell binding assays confirmed that the aptamer‐conjugated gold nanoparticles could specifically target the cancer cells and did not react to the control cells which do not have the biomarker on the surface. The potential therapeutic effect of the “nanobomb” generated by the aptamer‐conjugated gold nanoparticles on cancer cells is under investigation now and results will be discussed.