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Effect of Vasopressin on the renal distribution of Annexin A2 in Brattleboro rats
Author(s) -
Kaminski Henriette Josephine,
Dietrich Anja,
Mutig Kerim,
Mrowka Ralf,
Bachmann Sebastian,
Paliege Alexander
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1038.14
Subject(s) - annexin a2 , vasopressin , medicine , endocrinology , aquaporin 2 , antibody , biology , annexin , chemistry , microbiology and biotechnology , immunology , flow cytometry , mechanical engineering , water channel , engineering , inlet
Aim of the present study was to elucidate the renal expression pattern of Annexin A2 (ANXA2) during steady state and during activation of the urine concentration mechanism. To this end, adult Brattleboro rats (DI rats) were treated for 3d with the selective V2 vasopressin receptor agonist desmopressin (dDAVP, 5ng/h) or vehicle via osmotic minipump. Renal ANXA2 distribution was studied by double‐labelling immunofluorescence using two different antibodies against ANXA2 and antibodies against rat endothelial cell antigen for endothelial cells, Tamm‐Horsfall protein for thick ascending limbs (TAL) and aquaporin‐2 for collecting ducts (CD). In untreated DI rats strong ANXA2 immunoreactivity was detected in cells of the Bowman capsule and in endothelial cells. Cells of the CD showed intermediate staining throughout the cytosol. In addition we observed a weak but consistent ANXA2 signal in the apical membrane of TAL profiles. Treatment of DI rats with dDAVP caused a marked shift of ANXA2 protein from the cytosol of CD cells to the apical and the basolateral membrane. In summary, we have shown that ANXA2 is expressed in cells of the Bowman capsule, the renal endothelium, the TAL and in the collecting duct where it may be involved in vasopressin signalling. This study was supported by the German Research Foundation (FOR 667).