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Effect of Flavonoids and Low Level Pesticide Exposure on Copper and Zinc Homeostasis in Rats
Author(s) -
Bebe Frederick N.,
Panemangalore Myna
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.563.11
Subject(s) - chemistry , zinc , kidney , zoology , bioavailability , organophosphate , homeostasis , pesticide , copper , toxicity , selenium , endocrinology , medicine , food science , pharmacology , biology , agronomy , organic chemistry
The protective effect of flavonoids against disease is well‐documented, but little is known of their anti‐nutritional effects and potential interaction with pesticides. Two 30/d experiments (36 male SD rats each) were conducted to determine effect of administering flavonoid mixture (FM) with concomitant exposure to a pesticide mixture (PM) or FM at varied levels on changes in concentrations of zinc and copper in tissues. Experiment1: PM=chlorpyrifos, endosulfan and thiram at 25% LD 50 was dissolved in SB oil and gavage‐fed 0.1mL 5days/wk; FM in SB oil was mixed in AIN‐93M diet at 1.0 and 5.0 mM/kg diet and fed at libitum. Groups = Control (CON), PM, FM1, FM5, PM+FM1 and PM+FM5. Experiment 2: FM in 1% polyethylene glycol was gavage‐fed (0.5mL/5 days/wk), at increasing levels(mg/kg bw): Groups = CON, FM5, FM25, FM50, FM100 and FM200. Experiment 1: PM plus FM increased tissue Cu as compared to the CON by: plasma16‐37%, liver (9‐20%), kidney (13‐41%), small intestinal mucosa (IM) 41% and small intestines (SI) 77%. Experiment 2: FM fed at higher levels decreased tissue Cu by: plasma 22‐36%, liver (23%), kidney (24‐44%), IM (17‐26%), SI (58 & 77%) as compared to respective CON. Kidney zinc increased at higher concentrations of FM by 14‐34%. These results indicate that flavonoids can modify copper homeostasis depending on whether they are fed in the diet or by gavage. (USDA#KYX‐50‐03‐17H).