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Phenotypes of NUDIX Hydrolases
Author(s) -
Bianco Luiza,
AbdulKhaliq Sakinah,
Kulakowski Nicolette,
Hynes Thomas,
Kane Colleen,
Jones Cara,
O'Handley Suzanne
Publication year - 2021
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2021.35.s1.04546
Subject(s) - hydrolase , integrases , biology , complementation , superfamily , phenotype , genetics , enzyme , gene , biochemistry , plasmid
Enzymes of the Nudix Hydrolase superfamily are characterized by the ability to hydrolyze substrates containing nu cleoside di phosphate linked to some moiety x , hence the acronym Nudix. We are systematically analyzing the E. coli Nudix hydrolase knockouts for phenotypes. MutT is an established antimutator, and we have determined that the other Nudix hydrolases from E. coli are not antimutators. Complementation studies with Nudix hydrolases from M. tuberculosis have been carried out to determine which, if any, are antimutators; thus far, none have been determined to be antimutators. We also screened the E. coli Nudix hydrolase knockouts for antibiotic susceptibility with mixed results that are being analyzed further.