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The function of noncoding RNA BC200 in a human disease
Author(s) -
shin heegwon,
lee jungmin,
kim youngmi,
lee younghoon
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.525.10
Subject(s) - rna , gene knockdown , motility , ribosomal rna , biology , ribosome , messenger rna , hela , cell , non coding rna , gene , microbiology and biotechnology , genetics
Although BC200 RNA was originally identified as a neuron‐specific non‐coding RNA, it is overexpressed in various cancer tissues. Recently, BC200 RNA was shown to promote metastasis in several cancer cell lines, but its mechanism was not understood in detail. To determine this mechanism, we knocked down BC200 RNA in a cervical cancer cell line, HeLa, which overexpress the RNA, and examined cell motility, profiling of ribosome footprints, and the correlation between cell motility changes and genes exhibiting altered ribosome profiles. We found that knockdown of BC200 RNA decreased cell motility and altered more than 2‐fold expressions of 29 genes including S100A11 (which showed a reduced ribosome footprint) previously shown to increase cell motility. Knockdown of BC200 RNA significantly destabilized S100A11 mRNA, suggesting that the down‐regulation of S100A11 was mainly caused by loss of stability of its mRNA. Together, our results show that BC200 RNA increases cell motility by stabilizing S100A11 transcripts. Support or Funding Information This study was supported by National Research Foundation of Korea (NRF) funded by the Korea government (MSIP) (2017R1A6A3A11031308), and the KAIST High Risk High Return Project (HRHRP). This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .