3′ End labelling of RNA with32P suitable for rapid gel sequencing | Zendy

Greg Winter | Zendy; G.G. Brownlee | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

3′ End labelling of RNA with³²P suitable for rapid gel sequencing

Author(s) -

Greg Winter,

G.G. Brownlee

Publication year - 1978

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/5.9.3129

Subject(s) - biology , labelling , rna , transfer rna , biochemistry , microbiology and biotechnology , nucleotide , periodate , gene

A new general method of labelling the 2',3'-diol end of RNA with 32P has been devised suitable for gel sequencing. Poly(A) polymerase (E. coli) is incubated with the RNA and limiting amounts of alpha-32P-ATP. The mono-addition product is then cleaved with periodate and beta-eliminated with aniline, leaving the RNA terminally labelled with 3' 32P-phosphate. When applied to a model compound, tRNAPhe from E. coli, over 28 residues could be read from the 3' end.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research