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Human origin recognition complex binds to the region of the latent origin of DNA replication of Epstein–Barr virus
Author(s) -
Schepers Aloys,
Ritzi Marion,
Bousset Kristine,
Kremmer Elisabeth,
Yates John L.,
Harwood Janet,
Diffley John F. X.,
Hammerschmidt Wolfgang
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.16.4588
Subject(s) - biology , genetics , dna , dna replication , origin recognition complex , virology , virus , origin of replication , replication (statistics) , viral replication , gammaherpesvirinae , computational biology , eukaryotic dna replication , herpesviridae , viral disease
Epstein–Barr virus (EBV) replicates in its latent phase once per cell cycle in proliferating B cells. The latent origin of DNA replication, oriP , supports replication and stable maintenance of the EBV genome. OriP comprises two essential elements: the dyad symmetry (DS) and the family of repeats (FR), both containing clusters of binding sites for the transactivator EBNA1. The DS element appears to be the functional replicator. It is not yet understood how oriP ‐dependent replication is integrated into the cell cycle and how EBNA1 acts at the molecular level. Using chromatin immunoprecipitation experiments, we show that the human origin recognition complex (hsORC) binds at or near the DS element. The association of hsORC with oriP depends on the DS element. Deletion of this element not only abolishes hsORC binding but also reduces replication initiation at oriP to background level. Co‐immunoprecipitation experiments indicate that EBNA1 is associated with hsORC in vivo . These results indicate that oriP might use the same cellular initiation factors that regulate chromosomal replication, and that EBNA1 may be involved in recruiting hsORC to oriP .