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The tight junction protein ZO‐1 and an interacting transcription factor regulate ErbB‐2 expression
Author(s) -
Balda Maria S.,
Matter Karl
Publication year - 2000
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/19.9.2024
Subject(s) - tight junction , biology , microbiology and biotechnology , paracellular transport , transcription factor , claudin , promoter , transfection , gene expression , gene , genetics , permeability (electromagnetism) , membrane
Epithelial tight junctions regulate paracellular diffusion and restrict the intermixing of apical and basolateral plasma membrane components. We now identify a Y‐box transcription factor, ZONAB ( ZO ‐1‐associated n ucleic a cid‐ b inding protein), that binds to the SH3 domain of ZO‐1, a submembrane protein of tight junctions. ZONAB localizes to the nucleus and at tight junctions, and binds to sequences of specific promoters containing an inverted CCAAT box. In reporter assays, ZONAB and ZO‐1 functionally interact in the regulation of the ErbB‐2 promoter in a cell density‐dependent manner. In stably transfected overexpressing cells, ZO‐1 and ZONAB control expression of endogenous ErbB‐2 and function in the regulation of paracellular permeability. These data indicate that tight junctions directly participate in the control of gene expression and suggest that they function in the regulation of epithelial cell differentiation.