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Organization of DNA replication origins in the fission yeast genome
Author(s) -
Gómez María,
Antequera Francisco
Publication year - 1999
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/18.20.5683
Subject(s) - biology , control of chromosome duplication , origin recognition complex , dna replication , genetics , origin of replication , schizosaccharomyces , pre replication complex , schizosaccharomyces pombe , gene , licensing factor , replication timing , genome , eukaryotic dna replication , saccharomyces cerevisiae
Eukaryotic DNA replication initiates at multiple points along the chromosomes known as replication origins (ORIs). We have developed a strategy to identify ORIs directly from replication intermediates in the fission yeast Schizosaccharomyces pombe . Mapping of a selection of the novel ORIs onto the genome reveals their preferential localization at intergenic regions upstream from genes. These results are supported by the observation that a large proportion of regions overlapping gene promoters contain active ORIs. Mapping of the genomic ars1 replication origin at nucleotide resolution shows that replication initiates at a defined position immediately upstream from the hus5 + promoter. Deletion analysis indicates that the regulatory elements required to initiate transcription and replication lie in close proximity, suggesting a possible relationship between both processes in vivo .