A physico-chemical study of triple helix formation by an oligodeoxythymidylate with N3'->P5' phosphoramidate linkages | Zendy

B.-w. Zhou-Sun | Zendy; J. Liquier | Zendy; E. Taillandier | Zendy; Js Sun | Zendy; T. Garestier | Zendy; C. Hélène | Zendy; Sergei Gryaznov | Zendy

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A physico-chemical study of triple helix formation by an oligodeoxythymidylate with N3'->P5' phosphoramidate linkages

Author(s) -

B.-w. Zhou-Sun,

J. Liquier,

E. Taillandier,

Js Sun,

T. Garestier,

C. Hélène,

Sergei Gryaznov

Publication year - 1997

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/25.9.1782

Subject(s) - phosphoramidate , biology , triple helix , helix (gastropod) , biochemistry , genetics , zoology , gastropoda

Non-denaturing gel retardation assay, DNA melting experiments and FTIR spectroscopy were used to characterize the triple helix formed by a 15mer 2'-deoxythymidylate with N3'-->P5'phosphoramidate linkages with its target sequence. The results indicate that: (i) the pentadecadeoxythymidylate with phosphoramidate linkages [dT15(np)] is highly potent to form a triple helix with a dT15*dA15target duplex through Hoogsteenbase-pairing; (ii) it forms a dT15(np)*dA15xdT15(np) triplex with the single-stranded oligo-2'-deoxyadenylate (dA15) without detectable double-helical intermediate; (iii) it does not only form a triple helix on the dT15*dA15target duplex, but also partially displaces the dT15 strand from the dT15*dA15duplex to form a dT15(np)*dA15xdT15(np) complex.

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