Open AccessEcological approach to the use of secondary products of pea flour and rice grain processing into protein concentrates and phytin
Author(s) -
Ruzaliya Ulanova,
Денис Куликов,
В.А. Гулакова,
A G Ahremko,
М.И. Сложенкина,
В. В. Колпакова
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/848/1/012106
Subject(s) - food science , bran , single cell protein , amino acid , chemistry , biomass (ecology) , bioconversion , composition (language) , leucine , methionine , lysine , biology , fermentation , agronomy , biochemistry , raw material , organic chemistry , linguistics , philosophy
The process of bioconversion of the secondary product of pea flour processing into protein concentrate (serum) into fodder microbial-plant concentrate has been optimized. For this, a composition was selected from the culture of the fungus Geotrichium candidum 977 and the yeast Saccharamyces cerevisiae 121, a mathematical model of concentrate synthesis was developed in the form of an equation that adequately describes the dependence of the biomass yield on technological parameters: pH of the medium, temperature and amount of inoculum. The concentrate from the biomass had a protein mass fraction of 61.68 % of DS, from the biomass with the culture liquid - 57.90 %. Concentrates - biologically complete, the rate of essential amino acids was 107-226 %, out of 30 fatty acids, 97 % were acids that are part of animal fats, vegetable oils or marine organisms. The ratio of saturated and unsaturated acids is 1:3, the content of trans isomers is 5.1 %, omega-6 fatty acids (linoleic) is 19.73 %. The ability of symbiosis between the yeast S. cerevisiae 84/5 and the fungus Trich. cutaneum 656 has been proven. transform the components of whey remaining after the extraction of phytin from rice bran into protein biomass. The ratio of monocultures by mass fraction is 1:1, pH - 5.0…6.0, duration of growth - 72 hours, digestibility – 90 %. The protein is enriched with methionine, isoleucine, leucine, lysine. The amount of essential acids is 18-21 % higher than in concentrates obtained from individual monocultures. The use of concentrates is advisable to use in the diet of animals.