Open AccessSilencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo
Author(s) -
В. А. Черенко,
Е. А. Филипенко,
Т. С. Голубева
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/723/2/022041
Subject(s) - nicotiana benthamiana , rna silencing , rna interference , gene silencing , rna , rnase p , biology , ribonuclease iii , microbiology and biotechnology , virology , gene , biochemistry , virus
RNA interference (RNAi) using exogenous double-stranded RNA (dsRNA) has been used to silence the model gene of Nicotiana benthamiana phytoendesaturase. Here we report on an efficient technique for dsRNA synthesis using E. coli HT115 strain. This strain is deficient in RNase III, an enzyme that normally destroys most dsRNA in a bacterial cell and has been engineered to produce big quantities of dsRNA. We also used root treatment for dsRNA delivery to N. benthamiana plants. We found this method to be one of the most efficient ways to deliver dsRNA for plants.