Open AccessIn vitro responses of Areca catechu immature embryos on medium containing plant growth regulators
Author(s) -
Diny Dinarti,
Sudarsono Sudarsono,
Jekki Irawan
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/694/1/012026
Subject(s) - areca , catechu , nut , micropropagation , biology , explant culture , murashige and skoog medium , germination , horticulture , juglans , botany , traditional medicine , in vitro , medicine , biochemistry , structural engineering , engineering
Betel (Pinang) nuts are used as herbs, traditional medicine, and in the beverage industry. Planting materials for Areca nut cultivation are seeds. However, the presence of 2 – 3 months dormancy hampers germination of Areca nut seeds. Therefore, developing alternative strategies to overcome such problems is necessary. This study evaluates Areca nut immature embryo in vitro responses on MS medium having various plant growth regulators. The experiments were set up using a single factor in a complete randomized design. The embryos were planted either on Murashige and Skoog (MS) medium (MS0), MS with a combination of BAP (1 ppm) and NAA (0.1 ppm), or MS medium supplemented with GA3 (3 ppm). Areca nut immature embryos planted on a medium containing BAP (1 ppm) and NAA (0.1 ppm) than that on a medium containing GA3 (3 ppm). The germinated embryos were best on medium supplemented with a combination of BAP (1 ppm) and NAA (0.1 ppm). Immature Areca nut embryos planted on MS0 medium were unable to grow. In conclusion, Areca nut immature embryos may be used as explants for this palm tree’s micropropagation, and the embryos germinated well on a medium containing BAP and NAA.