Preservation of bovine semen using egg yolk substituted with extract guava in water from yellow coconut on the semen quality and fertility of Bali cattle

The present study used the ratio of 80% coconut water and 11 - 20% egg yolk substitution with extract guava ranged from 4 to 9% in order to determine the quality and fertility of Bali cattle storaged at 5 0 C. This study used a complete factorial design with two factors and six treatments. The first factor was coconut water and egg yolk substituted with extract guava extender at different ratio consisted of 6 treatments. They were: (1) T 0 (control) = 80% coconut water + 20% egg yolk without extract guava; (2) T 1 = 80% coconut water + 16% egg yolk + 4% extract guava; (3) T 1 = 80% coconut water + 15% egg yolk + 5% extract guava; (4) T 2 = 80% coconut water + 14% egg yolk + 6% extract guava; (5) T 3 = 80% coconut water + 13% egg yolk + 7%; and (5) T 4 = 80% coconut water + 12% egg yolk + 8% extract guava; and (6) T 5 = 80% coconut water + 11% egg yolk + 9% extract guava. The second factor was the length of storage at low temperature which consisted of 3 treatments, including 1 day, 2 days, 3 days, 4 days, 5 days, 6 days and 7 days. The semen used in this study had motility ranged from 50 to 55%. The variables measured in this study were intact plasma membrane (IPM) and intact acrosome hood (ICH). The results showed that the percentage of spermatozoa motility for T 1 until the sixth day of storage was 55.51%, T 2 until the seventh day of storage was 40.14%, T 0 to the fifth day was 41.73%, T 3 and T 4 until the fourth day was 43.85% and 41.11%, respectively, whereas for T 5 only until the third day were 51.00% and T 6 until the second day amounted to 48.83%. Viability of Bali cattle spermatozoa in the six treatment groups where egg yolk substituted with extract guava was higher (T 1 , T 2 and T 6 ) compared to T 0 from the first day until the fifth day of storage. It can be concluded that substitution of 15% egg yolk with 5% extract guava in 80% coconut water diluents can maintain IPM and ICH spermatozoa of Bali cattle until the sixth day of storaged at 5°C.