Direct Polymerase Chain Reaction for Detection of ToxigenicCorynebacterium diphtheriaeStrains from the Republic of Georgia after Prolonged Storage | Zendy

Ketevan Kobaidze | Zendy; Tanja Popović | Zendy; Hiroshi Nakao | Zendy; Linda Quick | Zendy

Open Access

Direct Polymerase Chain Reaction for Detection of ToxigenicCorynebacterium diphtheriaeStrains from the Republic of Georgia after Prolonged Storage

Author(s) -

Ketevan Kobaidze,

Tanja Popović,

Hiroshi Nakao,

Linda Quick

Publication year - 2000

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1086/315555

Subject(s) - corynebacterium diphtheriae , polymerase chain reaction , diphtheria toxin , diphtheria , corynebacterium , microbiology and biotechnology , throat , biology , virology , toxin , gene , bacteria , genetics , vaccination , anatomy

A total of 226 paired nose and throat swab specimens from 113 clinical diphtheria cases from the republic of Georgia were analyzed by direct polymerase chain reaction targeting both A and B subunits of the diphtheria toxin gene, tox. Even after prolonged transport and extensive storage (7-14 months) of the clinical specimens in silica gel packages, direct polymerase chain reaction detected the diphtheria tox gene in 54% of the specimens. Specimens obtained by throat swab were three times more likely than those obtained by nose swab to be positive for Corynebacterium diphtheriae.

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