Open AccessUpdating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility
Author(s) -
Witwer Kenneth W.,
Soekmadji Carolina,
Hill Andrew F.,
Wauben Marca H.,
Buzás Edit I.,
Di Vizio Dolores,
FalconPerez Juan M.,
Gardiner Chris,
Hochberg Fred,
Kurochkin Igor V.,
Lötvall Jan,
Mathivanan Suresh,
Nieuwland Rienk,
Sahoo Susmita,
Tahara Hidetoshi,
Torrecilhas Ana Claudia,
Weaver Alissa M.,
Yin Hang,
Zheng Lei,
Gho Yong Song,
Quesenberry Peter,
Théry Clotilde
Publication year - 2017
Publication title -
journal of extracellular vesicles
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.94
H-Index - 68
ISSN - 2001-3078
DOI - 10.1080/20013078.2017.1396823
Subject(s) - extracellular vesicles , reproducibility , extracellular vesicle , vesicle , computer science , computational biology , chemistry , microbiology and biotechnology , microvesicles , biology , biochemistry , chromatography , microrna , membrane , gene
An editorial describing “minimal experimental requirements for definition of extracellular vesicles (EVs)”, or more simply “minimal information for studies of EVs (MISEV)” was published in the Journal of Extracellular Vesicles in late 2014 [1]. Similar to guidelines in other scientific fields [2–4], “MISEV2014”, as we will call it here, provided recommendations on experimental methods and minimal information in reporting. Specifically, three key areas were addressed: EV isolation/purification, EV characterization and EV functional studies (see Text Box 1).