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High‐level expression of human interleukin‐17 in the yeast Pichia pastoris
Author(s) -
Zhou Limei,
Wang Jiaxi,
Peng Shangyun,
Duan Jubao,
Cai Xin,
Zou Minji,
SU Qing
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204662
Subject(s) - pichia pastoris , yeast , pichia , microbiology and biotechnology , secretion , receptor , gene , biology , chemistry , recombinant dna , biochemistry
Human interleukin‐17 (hIL‐17) gene without the signal sequence was isolated from activated peripheral blood lymphocytes by RT‐PCR, then highly expressed in the yeast Pichia pastoris in the form of the glycosylated monomer. The monomer of rhIL‐17 stimulated mouse fibroblast 3T3 cells to secrete IL‐6 and was specifically bound to its receptors on 3T3 cells.