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Degradation of transferrin and albumin by radical reactions in human plasma evaluated by immunoblot
Author(s) -
Yamada Yasuko,
Okamoto Eiko,
Tanaka Kyoko,
Kojo Shosuke
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204272
Subject(s) - transferrin , degradation (telecommunications) , human albumin , human plasma , chemistry , albumin , serum albumin , biochemistry , chromatography , computer science , telecommunications
Immunoblot analysis showed that transferrin and human serum albumin were degraded in a similar rate on the basis of the protein weight by radical reactions using a solution containing each protein initiated by Cu2+ and hydrogen peroxide. When plasma was treated with Cu2+ and hydrogen peroxide, the rate profile of the degradation of transferrin was similar to that of albumin based on immunoblot analysis in spite that the content of these proteins in plasma was considerably different. These results suggest that highly reactive hydroxyl radicals attack these proteins indiscriminately. Present observations demonstrate that immunoblot analysis is an effective method to analyze radical reactions of each protein in the presence of many other proteins like plasma.