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Regulation of Na,K‐ATPase activity in renal basolateral membrane of 1‐clip‐1‐kidney hypertensive rat
Author(s) -
Seok JeongHo,
Kim JuByoung,
Hong JangHee,
Sung JiYeon,
Hur GangMin,
Lim Kyu,
Lee JaeHeun
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204192
Subject(s) - ouabain , cycloheximide , endocrinology , medicine , kidney , renal cortex , messenger rna , chemistry , atpase , biology , sodium , microbiology and biotechnology , enzyme , biochemistry , gene , protein biosynthesis , organic chemistry
Abstract The changes of Na,K‐ATPase activity and its regulation have been investigated in the renal cortex of 1‐clip‐1‐kidney hypertensive rat. Ouabain‐sensitive Na,K‐ATPase activity (Emax) and [3H]ouabain‐binding site (Bmax) in the hypertensive rat were slightly increased than those in the control. The levels of Na,K‐ATPase α1‐ and β1‐subunit mRNA of the renal cortex in hypertensive rat were more increased than those in the control. Their increases were repressed by actinomycin‐D, but not altered or more increased by cycloheximide. These results suggest that the increase of Na,K‐ATPase activities and ouabain binding sites in 1‐clip‐1‐kidney hypertensive rat may be correlated with the increases of gene expression in transcription level and/or of mRNA stability of Na,K‐ATPase.