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Inhibition of mitochondrial gene expression by antisense RNA of mitochondrial transcription factor A (mtTFA)
Author(s) -
Inagaki Hidetoshi,
Kitano Shigetomo,
Lin Kong Hua,
Maeda Sumio,
Saito Takao
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202962
Subject(s) - tfam , mitochondrial dna , biology , microbiology and biotechnology , gene , transcription (linguistics) , protein subunit , gene expression , mitochondrion , genetics , linguistics , philosophy
Mitochondrial transcription factor A (mtTFA) plays an important role in regulating the expression of mitochondrial genes. To gain a better understanding of the relationships between mitochondrial gene expression and mtTFA, the mtTFA gene was inserted into a mammalian expression vector, both in the sense orientation and in the antisense orientation. After construction, these plasmids were transfected into COS‐7 cells. In antisense‐transformed cells, the expression of the cytochrome c oxidase subunit I and subunit III genes encoded by mitochondrial DNA was inhibited and there was an accompanying reduction of the level of mtTFA protein. These results provide direct evidence that the expression of mitochondrial genes is under the control of mtTFA.