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Lucigenin reduction by nadph‐cytochrome P450 reductase and the effect of phospholipids and albumin on chemiluminescence
Author(s) -
Tsukamoto Maki,
Tampo Yoshiko,
Yonaha Masanori
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202482
Subject(s) - lucigenin , menadione , chemiluminescence , superoxide , chemistry , reductase , microsome , cytochrome , biochemistry , enzyme , chromatography
To assess lucigenin, a chemilumigenic probe, as a detector of superoxide anion in microsomes, NADPH oxidation, lucigenin disappearance, and chemiluminescence in a system including purified NADPH‐cytochrome P450 reductase were exmnined. NADPH oxidation was increased by adding lucigenin, and concurrently, its disappearance and oxygen consumption were also stimulated. Chemiluminescence, which is negligibly emitted in the presence of the reductase alone, was remarkably amplified with phospholipids and albumin. Menadione inhibited lucigenin disappearance resulting in suppression of chemiluminescence. Lucigenin chemiluminescence measured in microsomes appears not to reflect direct superoxide anion production from microsomal components and from quinones, such as menadione.