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Attenuated antigenicity of ribonucleoproteins modified by reactive oxygen species
Author(s) -
Waris Ghulam,
Alam Khurshid
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800202412
Subject(s) - antigenicity , ribonucleoprotein , reactive oxygen species , chemistry , oxygen , virology , biochemistry , biology , immunology , antigen , rna , organic chemistry , gene
Abstract Ribonucleoprotein (RNP) particles isolated from fresh goat fiver nuclei were exposed to hydroxyl radical which induced modification in the gross structure of RNP particles. To evaluate the effect of hydroxyl modification on the antigenic properties of RNP and possible role of ROS‐RNP in the initiation and development of SLE, enzyme immunoassays were carried out. SLE sera having high titre anti‐DNA antibodies showed enhanced binding to hydroxyl modified RNP particles in comparison to unmodified RNP particles. In competition assay none of the SLE sera or isolated IgG showed preference for ROS‐modified RNP particles over native RNP particles. These studies suggest that anti‐RNP autoantibodies observed in subpopulation of SLE patients are generated by some other intra‐or extracellular mechanisms and hydroxyl radical has probably no direct role in the initiation of antibodies.