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Attenuation of renomedullary phospholipase C isozyme, PLC‐δ1, in spontaneously hypertensive rats
Author(s) -
Lee KweonHaeng,
Cho YoungJin,
Cha Seok Ho,
Endou Hitoshi
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204551
Subject(s) - isozyme , medicine , phospholipase c , endocrinology , medulla , western blot , kidney , chemistry , biology , enzyme , biochemistry , gene , receptor
Abstract The distributional patterns of PLC isozymes within the kidney were investigated using spontaneously hypertensive rats (SHRs) and normotensive Wistar‐Kyoto (WKY) rats at 4 and 12 weeks of age. PLC‐β1, PLC‐β3 and PLC‐δ1 quantified by Western blot analysis, were present in the highest concentrations in the inner medulla of rats at both 4 and 12 weeks of age. On the other hand, PLC‐β4, PLC‐γ1 and PLC‐γ2 were distributed almost equally among the regions for the rats of both ages. When compared with WKY rats at 12 weeks of age, the amounts of PLC‐β1, PLC‐β3, PLC‐γ1, PLC‐γ2, and PLC‐δ1 in the inner medulla of SHRs were significantly lower, and the amount of PLC‐δ1 in the inner stripe of the outer medulla was also significantly lower. Even at the prehypertensive stage at 4 weeks of age, the inner medullary concentration of PLC‐δ1 was significantly lower in SHRs than WKY rats. These results suggest that PLC‐δ1 would play an important role in the development of hypertension.