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Partial restoration of inactivated ribosomes: role of the aldehyde group generated by RNA N‐glycosidase in the sarcin/ricin domain of 28S rRNA in ribosome
Author(s) -
Cheng LePing,
Liu WangYi
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700202781
Subject(s) - ricin , ribosome , ribosome inactivating protein , ribosomal rna , amino acid , biochemistry , 23s ribosomal rna , protein biosynthesis , biology , 5.8s ribosomal rna , rna , chemistry , gene , toxin
In this paper, we report more information on the important role of the aldehyde group in the sarcin/ricin domain of 28S rRNA in rat liver ribosome. We find D‐amino acids, amino acid derivatives having free amino group and two polyamines can also partially restore the activity of cinnamomin‐inactivated ribosomes. However, amino acid derivatives and a tripeptide with blocked amino group cannot. Neither sodium borohydride nor the L‐amino acids can restore the activity of ribosomes inactivated by α‐sarcin. These data demonstrate that partial restoration of the activity of the inactivated ribosome is indeed the result of the blockage of the aldehyde group. It reaches the conclusion that emergence of the active aldehyde group in the sarcin/ricin domain of 28S rRNA is one of factors that inactivate the ribosome for protein synthesis.