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Effect of long time storage on cytosolic glutathione S‐transferases
Author(s) -
Peters Wilbert H. M.,
Roelofs Hennie M. J.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201961
Subject(s) - glutathione , cytosol , enzyme , isozyme , glutathione s transferase , transferase , biochemistry , glutathione transferase , chemistry , enzyme assay
Abstract Glutathione S‐transferases are a family of enzymes catalyzing the conjugation of glutathione to a wide variety of compounds. Stability of human liver cytosolic glutathione S‐transferase enzyme activity and levels of glutathione S‐transferase class Alpha and Mu were assessed. Cytosolic fractions of sixteen different patients were stored up to two years at three different temperatures: ‐20°, ‐80° and ‐196° Celsius. Samples were assayed for glutathione S‐transferase enzyme activity and glutathione S‐transferase class Alpha and Mu levels, after 3, 6, 12 and 24 months of storage. No significant differences in glutathione S‐transferase enzyme activity as well as in isoenzyme levels were found during this 24 months of storage at the three different temperatures. It is concluded that stability of glutathione S‐transferases allows long term storage even at ‐20°C, without significant loss of enzyme activity or enzyme protein.