Premium
Effect of Moderate Acute Exercise on Expression of mRNA Involved in the Calcineurin Signaling Pathway in Human Skeletal Muscle
Author(s) -
Hitomi Yoshiaki,
Kizaki Takako,
Katsumura Toshihito,
Mizuno Masao,
Itoh Chohei,
Esaki Kazuki,
Fujioka Yasunori,
Takemasa Tohru,
Haga Shukoh,
Ohno Hideki
Publication year - 2003
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216540310001592825
Subject(s) - calcineurin , nfat , mef2 , skeletal muscle , myocyte , endocrinology , medicine , signal transduction , messenger rna , gene expression , phosphatase , mef2c , biology , microbiology and biotechnology , enhancer , gene , biochemistry , transplantation , phosphorylation
Abstract Calcineurin, a calcium‐regulated protein phosphatase, activates gene expression specific to slow muscle fibers by dephosphorylating a family of the nuclear factor of activated T cells (NFAT), which cooperates with myocyte enhancer factor‐2 (MEF2) and AP‐1. However, it remains unknown how acute exercise influences this signaling pathway and leads to the development of slow muscle fibers. In the present study, we investigated the effect of moderate acute exercise on mRNA expression of genes in the calcineurin signaling pathway in human skeletal muscle. Five healthy volunteers underwent 1 h bicycle ergometer at 50%VO 2 max, and vastus lateralis muscle biopsies were collected before and after exercise. Four hours after exercise, alterations in mRNA expression of NFAT 1 S‐ S3 were observed with a wide variety among subjects, while c‐fos mRNA was significantly induced in all subjects. By contrast, the expression of calcineurin, MEF2, and myocyte‐enriched calcineurin‐interacting protein 1 (MCIP1) remained unchanged. These results suggest that even moderate acute exercise may change mRNA expression of genes in the calcineurin‐signaling pathway. IUBMB Life, 55: 409‐413, 2003