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Differential Regulation of the Secretions of Matrix Metalloproteinase‐9 and Tissue Inhibitor of Metalloproteinases‐1 from Human Keratinocytes in Culture
Author(s) -
Kobayashi Takashi,
Hattori Shunji,
Nagai Yutaka,
Tajima Shingo
Publication year - 2000
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/152165400300001552
Subject(s) - matrix metalloproteinase , messenger rna , metalloproteinase , tissue inhibitor of metalloproteinase , western blot , northern blot , matrix metalloproteinase 3 , microbiology and biotechnology , chemistry , cell culture , gelatinase a , biology , biochemistry , gene , genetics
To investigate the pathophysiological roles of matrix metalloproteinase (MMP)‐9 and tissue inhibitor of metalloproteinases (TIMP)‐1 in the differentiation of keratinocytes, we used human keratinocytes in culture to examine the gelatinolytic activity of MMP‐2 and MMP‐9 by zymographic analysis, MMP‐9 mRNA by reverse transcriptase‐polymerase chain reaction, and TIMP‐1 mRNA by Northern blot analysis. Adding of calcium (1 mM) to the culture medium increased the activity of MMP‐9 and MMP‐9 mRNA without affecting MMP‐2 activity or the expression of TIMP‐1 mRNA. These results suggest that keratinocytes regulate the expressions of MMP‐9 and TIMP‐1 differently during the process of differentiation.